PE anti-rat CD3 antibody: r-phycoerythin (r-PE) conjugate

Contents: Phycoerythrin (PE) anti-rat CD3
Catalog Number: 12-0030
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioG4.18 (G4.18)
Isotype: Mouse IgG3, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-0030-81	50 ug	$95
12-0030-82	100 ug	$155
12-0030-83	200 ug	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of Lou rat splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 μg of PE anti-rat CD3 (G4.18) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0030	Fluorescein isothiocyanate (FITC) anti-rat CD3	488	518	FC
12-0030	Phycoerythrin (PE) anti-rat CD3	488	575	FC
13-0030	Biotin anti-rat CD3	N/A	N/A	FC
14-0030	Affinity Purified anti-rat CD3	N/A	N/A	FC IHC IP WB
16-0030	Functional Grade* Purified anti-rat CD3	N/A	N/A	FA FC IHC WB
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nicolls MR, Aversa GG, Pearce NW, Spinelli A, Berger MF, Gurley KE, Hall BM. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation. 1993 Mar;55(3):459-68. (G4.18, FC, IP, FA, PubMed)

Tran GT, Carter N, He XY, Spicer TS, Plain KM, Nicolls M, Hall BM, Hodgkinson SJ. Reversal of experimental allergic encephalomyelitis with non-mitogenic, non-depleting anti-CD3 mAb therapy with a preferential effect on T(h)1 cells that is augmented by IL-4. Int Immunol. 2001 Sep;13(9):1109-20. (G4.18, FC, FA, PubMed)

Upham JW, Strickland DH, Bilyk N, Robinson BW, Holt PG. Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion. Immunology. 1995 Jan;84(1):142-7.

Brenan M, Rees DJ. Sequence analysis or rat integrin alphaE1 and alphaE2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 2000 Jun;30(6):1527-37.

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