Product Information

Contents: Phycoerythrin (PE) anti-human CD2 (LFA-2)
Catalog Number: 12-0029
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RPA-2.10
Isotype: Mouse IgG1, κ
HLDA No.: IV T085

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or PE anti-human CD2 RPA-2.10 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0029	Affinity Purified anti-human CD2 (LFA-2)	N/A	N/A	FA FC IH/F WB
16-0029	Functional Grade* Purified anti-human CD2 (LFA-2)	N/A	N/A	FC
13-0029	Biotin anti-human CD2 (LFA-2)	N/A	N/A	FC
11-0029	FITC anti-human CD2 (LFA-2)	488	518	FC
12-0029	PE anti-human CD2 (LFA-2)	488	575	FC
15-0029	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD2 (LFA-2)	488	670	FC
17-0029	Allophycocyanin (APC) anti-human CD2 (LFA-2)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RPA-2.10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RPA-2.10 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Aversa GG, Bishop GA, Suranyi MG, Hall BM. (1987). RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation. Transplant Proc. 19:277-8

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Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control