Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human Perforin
Catalog Number: 11-9994
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: dG9 (delta G9)
Isotype: Mouse IgG2b, κ

Human PBMCs were surface-stained with PE anti-human CD56 (MEM188) (cat. 12-0569), then fixed and permeabilized and subsequently stained with FITC anti-human Perforin (delta G9) or FITC mouse IgG2b Iso Cntrl (cat. 11-4732). Quadrant lines demarcate the isotype control. Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-9994	Fluorescein isothiocyanate (FITC) anti-human Perforin	488	518	IC Flow IHC IP
12-9994	Phycoerythrin (PE) anti-human Perforin	488	575	IC Flow
14-9994	Affinity Purified anti-human Perforin	N/A	N/A	IHC IP

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested

This dG9 (delta G9) antibody has been tested by intracellular flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 20 μl (.125 μg) per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

M.J. Smyth, et al, (1990) J Exp Med 171: 1269-1281.
A. Hameed, et al, (1992) Am J Pathol 140: 1025-1030.
P.L. Jose, et al, (1994) J Immunol 148: 3354-3360.
B.C. Schlesinger & L. Cheng, (1994) Immunol 81: 291-295.
C.C. Liu, et al, (1995) Immunol Today 16: 194-201.

Related Products

Cat. 11-4732    FITC Mouse IgG2b Isotype Control
Cat. 88-7095    Human Perforin ELISA Ready-SET-Go!
Cat. 88-7395    Human Perforin ELISPOT Ready-SET-Go!
Cat. 11-9995    **PLEASE SEE NEW CLONE, CAT# 11-9994 INSTEAD** Fluorescein isothiocyanate (FITC) anti-human Perforin (clone 1B4)
Cat. 12-9995    **PLEASE SEE NEW CLONE, CAT# 12-9994 INSTEAD** Phycoerythrin (PE) anti-human Perforin (clone 1B4)
Cat. 14-9995    Affinity Purified anti-human Perforin (clone 1B4)