Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD235a (glycophorin A)
Catalog Number: 11-9987
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIR2 (also GA-R2)
Isotype: Mouse IgG2b, κ

Staining of normal human whole blood with 0.03 μg of FITC Mouse IgG2b Iso Cntrl (cat. 11-4732) (open histogram) or 0.03 μg of FITC anti-human CD235a (HIR2) (colored histogram). Cells in the erythrocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9987	Affinity Purified anti-human CD235a (glycophorin A)	N/A	N/A	FC
13-9987	Biotin anti-human CD235a (glycophorin A)	N/A	N/A	FC
11-9987	FITC anti-human CD235a (glycophorin A)	488	518	FC
12-9987	PE anti-human CD235a (glycophorin A)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIR2 monoclonal antibody reacts with human glycophorin A and B, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at less than or equal to 0.03 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

Related Products

Cat. 11-4732 FITC Mouse IgG2b Isotype Control