mouse AID antibody, Fluorescein isothiocyanate (FITC) conjugate

Contents: Fluorescein isothiocyanate (FITC) anti-mouse AID (Activation-induced cytidine deaminase)
Catalog Number: 11-9984
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio911
Isotype: Rat IgG2a, κ

Product Options

Price

Cat. No.

Size

To view relevant pricing,
please select your country
and resume browsing.

11-9984-80

25 ug

11-9984-82

100 ug

Staining of mouse AID-transiently transfected HEK 293T cells with 0.5 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (open histogram) or 0.5 μg of FITC anti-mouse AID (eBio911) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9984	Affinity Purified anti-mouse AID (Activation-induced cytidine deaminase)	N/A	N/A	WB
11-9984	Fluorescein isothiocyanate (FITC) anti-mouse AID (Activation-induced cytidine deaminase)	488	518	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio911 antibody reacts with mouse activation-induced cytidine deaminase (AID), a member of the cytidine deaminase family, which was cloned from a mouse B-cell lymphoma line induced by combined stimulation with transforming growth factor-beta, interleukin-4, and CD40 ligand. The human AID gene encodes a 198-amino acid protein containing a conserved cytidine deaminase motif. The human AID protein shares 92% amino acid identity with mouse AID. RT-PCR analysis of 15 human tissues detected strong expression of AID mRNA in lymph nodes and tonsils.
Class switch recombination (CSR) is a region-specific DNA recombination reaction that replaces one immunoglobulin (Ig) heavy-chain constant region gene with another. This enables a single variable region gene to be used in conjunction with different downstream heavy-chain constant region genes, each having a unique biologic activity. AID, a putative RNA editing enzyme, is required for this action.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio911 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBio911 antibody has been tested by intracellular staining and flow cytometric analysis of mouse AID-transfected HEK 293T cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Muramatsu, M., V.S. Sankaranand, S. Anant, M. Sugai, K. Kinoshita, N.O. Davidson, and T. Honjo. 1999. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 274: 18470-18476.

Kinoshita, K., M. Harigai, S. Fagarasan, M. Muramatsu, and T. Honjo. 2001. A hallmark of active class switch recombination: transcripts directed by I promoters on looped-out circular DNAs. PNAS. 98: 12620-12633.

Nagaoka H., M. Muramatsu, N. Yamamura, K. Kinoshita, and T. Honjo. 2002. Activation-induced deaminase (AID)-directed hypermutation in the immunoglobulin Sµ region: implication of AID involvement in a common step of class switch recombination and somatic hypermutation. J. Exp. Med. 195: 539-534.

Related Products

Cat. 11-4321 FITC Rat IgG2a Isotype Control