Staining of unstimulated (left) or 3-day ConA activated (right) BALB/c splenocytes with 0.5 µg of FITC Armenian hamster IgG isotype control (cat.11-4444) (open histogram) or 0.25 µg of FITC C398.4A (colored histogram). Total viable cells were used for analysis.
Contents: Fluorescein isothiocyanate (FITC) anti-mouse/rat ICOS (CD278) Catalog Number: 11-9949 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Clone: C398.4A Host/Isotype: Armenian Hamster IgG Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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Size
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11-9949-80
25 ug
11-9949-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The C398.4A mAb reacts with ICOS (Inducible COStimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e. C398.4A does not block binding of ICOSL).
Applications Reported
For research use only, not for diagnostic or therapeutic use. The C398.4A antibody has been reported for use in flow cytometric analysis.
Applications Tested
The C398.4A antibody has been tested by flow cytometric analysis of mouse ConA-activated (2 days) spleen cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Lucia, M. B., D. Buonfiglio, et al. 2000. Expression of the novel T cell activation molecule hpH4 in HIV-infected patients: correlation with disease status. AIDS Res Hum Retroviruses 16(6): 549-57. Buonfiglio, D., M. Bragardo, et al. 1999. Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas. Eur J Immunol 29(9): 2863-74. Redoglia, V., U. Dianzani, et al. 1996. Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor. Eur J Immunol 26(11): 2781-9. Arimura Y, Shiroki F, Kuwahara S, Kato H, Dianzani U, Uchiyama T, Yagi J. Akt is a neutral amplifier for Th cell differentiation. J Biol Chem. 2004 Mar 19;279(12):11408-16. Epub 2004 Jan 5. [activating]
