Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human ICOS (CD278)
Catalog Number: 11-9948
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: ISA-3
Isotype: Mouse IgG1, κ

Staining of 2-day unstimulated (left) and 2-day CD3/CD28 activated (right) human PBMC with FITC Mouse IgG1, K Iso Cntrl (cat. 11-4719) (open histogram) or FITC ISA-3 (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9948	Affinity Purified anti-human ICOS (CD278)	N/A	N/A	FA FC IH/F IP
16-9948	Functional Grade* Purified anti-human ICOS (CD278)	N/A	N/A	FA FC
13-9948	Biotin anti-human ICOS (CD278)	N/A	N/A	FC
11-9948	FITC anti-human ICOS (CD278)	488	518	FC
12-9948	Phycoerythrin (PE) anti-human ICOS (CD278)	488	575	FC
25-9948	Phycoerythrin-Cy7 (PE-Cy7) anti-human ICOS (CD278)	488	760	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (catalog # 14-9949), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948 or 12-9948 rather than 11-9948.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ISA-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The ISA-3 antibody has been pre-titrated and tested by flow cytometric analysis of unstimulated and CD3/CD28-activated (3 days) human blood leukocytes. This can be used at 20 μl per million cells in a 100 μl total staining volume.

References

Hutloff A, Dittrich AM, Beier KC, Eljaschewitsch B, Kraft R, Anagnostopoulos I, Kroczek RA. 1999. ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28. Nature. 397(6716):263-6.

Buonfiglio D, Bragardo M, Redoglia V, Vaschetto R, Bottarel F, Bonissoni S, Bensi T, Mezzatesta C, Janeway Jr CA, Dianzani U. 2000. The T cell activation molecule H4 and the CD28-like molecule ICOS are identical. Eur J Immunol. 30(12):3463-7.

Grimbacher B, Hutloff A, Schlesier M, Glocker E, Warnatz K, Drager R, Eibel H, Fischer B, Schaffer AA, Mages HW, Kroczek RA, Peter HH. 2003. Homozygous loss of ICOS is associated with adult-onset common variable immunodeficiency. Nat Immunol. 4(3):261-8.

Beier KC, Hutloff A, Dittrich AM, Heuck C, Rauch A, Buchner K, Ludewig B, Ochs HD, Mages HW, Kroczek RA. 2000. Induction, binding specificity and function of human ICOS. Eur. J. Immunol. 30, 3707.

Related Products

Cat. 11-4714  Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control
Cat. 11-9949  FITC anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 12-9949  PE anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 13-9949  Biotin anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 14-9949  Affinity Purified anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 15-9949  PE-Cy5 anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 16-9949  Functional Grade Purified anti-mouse/rat ICOS (CD278) (clone C398.4A)