Product Information

Contents: Fluorescein isothiocyanate (FITC) H-Y TCR (male antigen HY)
Catalog Number: 11-9930
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: T3.70
Isotype: Mouse IgG1, κ

Surface staining of CD11.3 cells with anti-mouse H-Y TCR (T3.70) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9930	Affinity Purified anti- H-Y TCR (male antigen HY)	N/A	N/A	FC
13-9930	Biotin anti- H-Y TCR (male antigen HY)	N/A	N/A	FC
11-9930	FITC anti- H-Y TCR (male antigen HY)	488	518	FC
12-9930	PE anti- H-Y TCR (male antigen HY)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The T3.70 monoclonal antibody reacts with the transgenic αβTCR that recognizes the male antigen H-Y in the context of H-2D^b. A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4⁺ and CD8⁺ T cell differentiation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The T3.70 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The T3.70 antibody has been tested by flow cytometric analysis of CD11.3 line, which is derived from a thymic tumor of the H-Y TCR transgenic mice and expresses the transgenic H-Y TCR. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Teh, H. S., H. Kishi, et al. (1990). "Early deletion and late positive selection of T cells expressing a male-specific receptor in T-cell receptor transgenic mice." Dev Immunol 1(1): 1-10.
Teh, H. S., H. Kishi, et al. (1989). "Deletion of autospecific T cells in T cell receptor (TCR) transgenic mice spares cells with normal TCR levels and low levels of CD8 molecules." J Exp Med 169(3): 795-806.
Teh, H. S., P. Kisielow, et al. (1988). "Thymic major histocompatibility complex antigens and the alpha beta T-cell receptor determine the CD4/CD8 phenotype of T cells." Nature 335(6187): 229-33.

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