FITC anti-mouse TLR2 (CD282, Toll-like receptor 2, TLR-2) antibody: Fluorescein conjugate

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Contents: Fluorescein isothiocyanate (FITC) anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)
Catalog Number: 11-9022
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: mT2.7
Isotype: Mouse IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
11-9022-80	25 ug	$105
11-9022-82	100 ug	$275

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with PE anti-mouse CD11b (M1/70) (cat. 12-0112) and 20 μL FITC Mouse IgG2a Iso Cntrl (cat. 11-4729) (left) or 0.25 μg FITC anti-mouse TLR2 (mT2.7) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-9022	Fluorescein isothiocyanate (FITC) anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	518	FC
12-9022	Phycoerythrin (PE) anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	488	575	FC
13-9022	Biotin anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC
14-9022	Affinity Purified anti-mouse Toll-like receptor 2 (CD282, TLR2, TLR-2)	N/A	N/A	FC IH/F IP

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The mT2.7 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). Mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This mT2.7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The mT2.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Meng G, Rutz M, Schiemann M, Metzger J, Grabiec A, Schwandner R, Luppa PB, Ebel F, Busch DH, Bauer S, Wagner H, Kirschning CJ. 2004. Antagonistic antibody prevents toll-like receptor 2-driven lethal shock-like syndromes. J Clin Invest. 2004. 113:1473-81.

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