Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse/human SLP-76 (SLP76)
Catalog Number: 11-7769
Sizes: 25 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HS76
Isotype: Mouse IgG2a

Staining of normal human peripheral blood cells with APC anti-human CD3 (UCHT1) (cat. 17-0038) and FITC Mouse IgG2a, K Iso Cntrl (cat. 11-4724) (left) or FITC anti-human SLP-76 (HS76) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7769	Affinity Purified anti-human SLP-76 (SLP76)	N/A	N/A	FC WB
11-7769	Fluorescein isothiocyanate (FITC) anti-human SLP-76 (SLP76)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HS76 antibody reacts with human SLP-76. SLP-76 (SH2 domain-containing leukocyte protein of 76 kD) has also been described as lymphocyte cytosolic protein 2 (LCP2 ) and 76-kDa phosphoprotein (pp76 ). SLP-76 is a cytosolic adaptor protein expressed in several cell types of hematopoietic origin. SLP-76 associates with a number of additional molecules and is required for coupling receptor ligation with more distal signaling events in immature thymocytes, mature T cells, platelets, and mast cells. SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HS76 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This HS76 antibody has been pre-titrated and tested by flow cytometric analysis of fixed and permeabilized normal human peripheral blood mononuclear cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Cao MY, Davidson D, Yu J, Latour S, Veillette A. 1999. Clnk, a novel SLP-76-related adaptor molecule expressed in cytokine-stimulated hemopoietic cells. J Exp Med 190:1527-1534.
Clements JL, Yang B, Ross-Barta SE, Eliason SL, Hrstka RF, Williamson RA, Koretzky GA. 1998. Requirement for the leukocyte-specific adapter protein SLP-76 for normal T cell development. Science 281:416-419.
Clements JL, Lee JR, Gross B, Yang B, Olson JD, Sandra A, Watson SP, Lentz SR, Koretzky GA. 1999. Fetal hemorrhage and platelet dysfunction in SLP-76-deficient mice. J Clin Invest 103:19-25.
Boerth, NJ, Sadler, JJ, Bauer, DD, Clements, JL, Gheith, SM, and GA Koretzky. Recruitment of SLP-76 to the membrane and glycolipid-enriched membrane microdomains replaces the requirement for linker for activation of T cells in T cell receptor signaling. J. Exp. Med. 192(7):1047, 2000.

Related Products

Cat. 11-4724  Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control
Cat. 11-7761  Fluorescein isothiocyanate (FITC) anti-mouse SLP-76 (SLP76) (clone MS76)
Cat. 14-7761  Affinity Purified anti-mouse SLP-76 (SLP76) (clone MS76)