Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CMKLR1 (serpentine chemokine-like receptor 1)
Catalog Number: 11-7581
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BZ332
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7581	Affinity Purified anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker)	N/A	N/A	FC
13-7581	Biotin anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker)	N/A	N/A	FC
11-7581	Fluorescein isothiocyanate (FITC) anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker)	488	518	FC
12-7581	Phycoerythrin (PE) anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker)	488	575	FC
51-7581	Alexa Fluor® 647 anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker)	633	668	FC
53-7581	Alexa Fluor® 488 anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The BZ332 antibody reacts with human chemokine-like receptor 1, CMKLR1. CMKLR1 (also known as ChemR23 or DEZ), is a recently-described serpentine chemoattractant receptor. CMKLR1 has been shown to be expressed by dendritic cells (DC) generated in vitro from monocytes, and to mediate their migration to the proteolytically regulated chemoattractant chemerin. CMKLR1 is expressed by circulating DCs in human blood. Importantly, it is selectively expressed by immature plasmacytoid DCs, but not myeloid DCs, thus enabling discrimination between these two dendritic cell lineages. While CMKLR1 is expressed by circulating pDCs in human blood, mDCs, as well as lymphocytes, monocytes, neutrophils, and eosinophils are negative. Human serum contains a chemoattractant for CMKLR1 which is identical with chemerin; this activity is triggered during blood coagulation, suggesting a mechanism for attraction of pDCs to sites of tissue damage and bleeding. Expression of CMKLR1 and migration to locally activated chemerin may allow the rapid and differential recruitment of pDCs in vivo. Unlike mDCs, circulating pDCs do not respond to inflammatory chemokines.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This BZ332 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This BZ332 antibody has been pre-titrated and tested by flow cytometric analysis of human CMKLR1-transfected cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Zabel, B., et al. 2005. Chemokine-Like Receptor 1 Expression and Chemerin-Directed Chemotaxis Distinguish Plasmacytoid from Myeloid Dendritic Cells in Human Blood. J. Immunol. 174: 244-251.

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