Contents: Fluorescein isothiocyanate (FITC) anti-human CMKLR1 (serpentine chemokine-like receptor 1)
Catalog Number: 11-7581
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BZ332
Isotype: Rat IgG2a, κ
| Prices for This Product* |
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*International customers: Please contact your distributor for region specific pricing.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-7581 |
Fluorescein isothiocyanate (FITC) anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker) |
488 |
518 |
FC
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| 12-7581 |
Phycoerythrin (PE) anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker) |
488 |
575 |
FC
|
| 51-7581 |
Alexa Fluor® 647 anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker) |
633 |
668 |
FC
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| 53-7581 |
Alexa Fluor® 488 anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker) |
488 |
519 |
FC
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| 14-7581 |
Affinity Purified anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker) |
N/A |
N/A |
FC
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| 13-7581 |
Biotin anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker) |
N/A |
N/A |
FC
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The BZ332 antibody reacts with human chemokine-like receptor 1, CMKLR1.
CMKLR1 (also known as ChemR23 or DEZ), is a recently-described serpentine chemoattractant receptor. CMKLR1 has been shown to be expressed by dendritic cells (DC) generated in vitro from monocytes, and to mediate their migration to the proteolytically regulated chemoattractant chemerin. CMKLR1 is expressed by circulating DCs in human blood. Importantly, it is selectively expressed by immature plasmacytoid DCs, but not myeloid DCs, thus enabling discrimination between these two dendritic cell lineages. While CMKLR1 is expressed by circulating pDCs in human blood, mDCs, as well as lymphocytes, monocytes, neutrophils, and eosinophils are negative. Human serum contains a chemoattractant for CMKLR1 which is identical with chemerin; this activity is triggered during blood coagulation, suggesting a mechanism for attraction of pDCs to sites of tissue damage and bleeding. Expression of CMKLR1 and migration to locally activated chemerin may allow the rapid and differential recruitment of pDCs in vivo. Unlike mDCs, circulating pDCs do not respond to inflammatory chemokines.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This BZ332 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This BZ332 antibody has been pre-titrated and tested by flow cytometric analysis of human CMKLR1-transfected cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Zabel, B., et al. 2005. Chemokine-Like Receptor 1 Expression and Chemerin-Directed Chemotaxis Distinguish Plasmacytoid from Myeloid Dendritic Cells in Human Blood. J. Immunol. 174: 244-251.
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