Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD45RB
Catalog Number: 11-7457
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MEM-55 (MEM55)
Isotype: Mouse IgG1
HLDA No.: III

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7457	Affinity Purified anti-human CD45RB	N/A	N/A	FC
11-7457	Fluorescein isothiocyanate (FITC) anti-human CD45RB	488	518	FC
12-7457	Phycoerythrin (PE) anti-human CD45RB	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MEM-55 monoclonal antibody reacts with human CD45RB, the 190, 205, and 220 kDa isoforms of the leucocyte common antigen (LCA). CD45RB is expressed on a subset of B cells, T cells, monocytes, and at lower density on granulocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MEM-55 (MEM55) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MEM-55 (MEM55) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control