Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse TNF-alpha (TNF-α)
Catalog Number: 11-7321
Sizes: 25 tests, 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MP6-XT22
Isotype: Rat IgG1, κ

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-mouse CD4 (GK1.5) and intracellularly stained with PE anti-mouse TNF-a (MP6-Xt22). (Right) The cells were surface stained with PE anti-mouse CD4 and intracellularly stained with APC anti-mouse TNF-a.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7321	FITC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	518	IC Flow
12-7321	PE anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	575	IC Flow
14-7321	Affinity Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IC Flow IH/F
16-7321	Functional Grade* Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	NU
17-7321	APC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	660	IC Flow
19-7321	Cy5 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	670	IC Flow
51-7321	Alexa Fluor® 647 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	668	IC Flow
53-7321	Alexa Fluor® 488 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	519	IC Flow
57-7321	Pacific Blue® anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MP6-XT22 antibody has been reported for use in ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested

The MP6-XT22 antibody is offered in 2 formats:

- μg size: has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 20 μl per million cells in a 100 μl total staining volume. The pre-titrated test size contains 0.2% BSA.

References

Hunter, C. A., M. J. Litton, et al. (1994). "Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis." J Infect Dis 170(4): 939-45.
Litton, M. J., B. Sander, et al. (1994). "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." J Immunol Methods 175(1): 47-58.
Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

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