Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human Interferon-γ (IFN-γ)
Catalog Number: 11-7319
Sizes: 25 tests, 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 4S.B3
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7319	Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	IC Flow
13-7319	Biotin anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA det ELISPOT
11-7319	FITC anti-human IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7319	PE anti-human IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
17-7319	APC anti-human IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
25-7319	Phycoerythrin-Cy7 (PE-Cy7) anti-human IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
51-7319	Alexa Fluor® 647 anti-human IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7319	Alexa Fluor® 488 anti-human IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7319	Pacific Blue® anti-human IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow
45-7319	PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g) (PE-Cy5.5 replacement)	488	690	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 4S.B3 monoclonal antibody reacts with interferon-γ (IFN-γ). The 4S.B3 antibody is a neutralizing antibody. Human IFN-γ is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 4S.B3 antibody has been reported for use in intracellular staining for flow cytometric analysis.

Applications Tested

This 4S.B3 antibody is offered in 2 formats:

- μg size: has been tested by intracellular flow cytometric analysis of activated human cells. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by intracellular flow cytometric analysis of activated human cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume). The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

References

Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon." J Interferon Res 4(4): 619-25.

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