Contents: Fluorescein isothiocyanate (FITC) anti-mouse IFN-γ
Catalog Number: 11-7311
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: XMG1.2
Isotype: Rat IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. (Left) The cells were surface-stained with PE anti-mouse CD4 (GK1.5) and intracellularly stained with FITC anti-mouse IFN-g (XMG1.2). (Right) The cells were surface stained with APC anti-mouse CD4 and intracellularly stained with PE anti-mouse IFN-g.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-7311 |
FITC anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
518 |
IC Flow
|
| 12-7311 |
PE anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
575 |
IC Flow
|
| 13-7311 |
Biotin anti-mouse IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
ELISA
|
| 14-7311 |
Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
ELISA cap
|
| 16-7311 |
Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
NU
|
| 17-7311 |
APC anti-mouse IFNg (Interferon-gamma, IFN-g) |
633 |
660 |
IC Flow
|
| 19-7311 |
Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g) |
633 |
670 |
IC Flow
|
| 25-7311 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
760 |
IC Flow
|
| 45-7311 |
PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
690 |
IC Flow
|
| 51-7311 |
Alexa Fluor® 647 anti-mouse IFNg (Interferon-gamma, IFN-g) |
633 |
668 |
IC Flow
|
| 53-7311 |
Alexa Fluor® 488 anti-mouse IFNg (Interferon-gamma, IFN-g) |
488 |
519 |
IC Flow
|
| 57-7311 |
Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g) |
402 |
421 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. XMG1.2 has been reported for use in intracellular staining with flow cytometric analysis.
Applications Tested
The FITC conjugated XMG1.2 is offered in 2 formats:
- µg size: has been tested by intracellular flow cytometric analysis and can be used at less than or equal to 0.5 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular flow cytometric analysis and can be used at 20 μl (0.25μg) per million cells in a 100 μl total staining volume. The pre-titrated test size contains 0.2% BSA.
References
Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples."
Immunol Rev 127: 5-24.
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