Fluorescein isothiocyanate (FITC) anti-mouse IL-17A (Interleukin-17A, IL17A)

Contents: Fluorescein isothiocyanate (FITC) anti-mouse IL-17A (Interleukin-17A, IL17A)
Catalog Number: 11-7177
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio17B7
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
11-7177-81	50 ug	$130

*International customers: Please contact your distributor for region specific pricing.

Intracellular staining of splenocytes cultured under Th17-polarizing conditions for 3 days followed by reactivation with PMA and Ionomycin in the presence of brefeldin A for 5 hours. Samples were stained with 0.125 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (left) or 0.125 μg of FITC anti-mouse IL-17A (eBio17B7) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7177	Fluorescein isothiocyanate (FITC) anti-mouse IL-17A (Interleukin-17A, IL17A)	488	518	IC Flow
12-7177	Phycoerythrin (PE) anti-mouse IL-17A (Interleukin-17A, IL17A)	488	575	IC Flow
13-7177	Biotin anti-mouse IL-17A (Interleukin-17A, IL17A)	N/A	N/A	ELISA det ELISPOT det
17-7177	Allophycocyanin (APC) anti-mouse IL-17A (Interleukin-17A, IL17A)	633	660	IC Flow
45-7177	PerCP-Cy5.5 anti-mouse IL-17A (Interleukin-17A)	488	690	IC Flow
51-7177	Alexa Fluor® 647 anti-mouse IL-17A (Interleukin-17A, IL17A)	633	668	IC Flow
53-7177	Alexa Fluor® 488 anti-mouse IL-17A (Interleukin-17A, IL17A)	488	519	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio17B7 antibody reacts with mouse IL-17A. The eBio17B7 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-γ and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio17B7 antibody has been reported useful for neutralization of mouse IL-17A, for ELISA/ELISPOT detection, and for intracellular staining for flow cytometric analysis.

Applications Tested

This eBio17B7 antibody has been tested by intracellular staining and flow cytometric analysis of PMA and Ionomycin-restimulated splenocytes cultured under Th17-polarizing conditions for 3 days. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Staining has been successfully done using the Foxp3 buffer system (cat 00-5523). Please contact Technical Support Department at tech@ebioscience.com.

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