Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human interleukin (IL-13)
Catalog Number: 11-7139
Sizes: 25 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: PVM13-1
Isotype: Mouse IgG1

Purified human CD4+ cells were stimulated with immobilized anti-CD3, soluble anti-CD28, IL-2, and IL-4 for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with PMA and Ionomycin in the presence Brefeldin A for 4 hours. (Left) The cells were surface stained with PE anti-human CD4 (RPA-T4) and intracellularly stained with FITC mouse IgG1 Isotype control. (Right) The cells were surface stained with PE anti-human CD4 and intracellularly stained with FITC anti-human IL-13 (PVM13-1).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7139	Fluorescein (FITC) anti-human IL-13 (Interleukin-13, IL13)	488	518	IC Flow
14-7139	Affinity Purified anti-human IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA cap ELISPOT IC Flow NU
16-7139	Functional Grade* Purified anti-human IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISPOT cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The PVM13-1 antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8⁺ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The PVM13-1 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested

This PVM13-1 antibody has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 5 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Griffioen, M., et al. 2004. Cancer Immunol. Immunother. 53: 715-722.
Van Halteren, A.G., et al. 2002. Diabetes 51: 2119-2125.
Martin, S., et al. 2001. N. Engl. J. Med. 345: 1036-1040.
Raz, I., et al.. 2001. Lancet 358: 1749-1753.
Schloot, N., et al. 2003. J. Autoimmun. 21: 365-376.

Related Products

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