Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse Interleukin-6 (IL-6)
Catalog Number: 11-7061
Sizes: 25 tests, 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MP5-20F3
Isotype: Rat IgG1, κ

Mouse thiogylcolate induced PEC were stimulated with LPS in the presence of Brefeldin A for 6 hours. The cells were surface stained with FITC anti-mouse F4/80 antigen (BM8) and intracellularly stained with PE anti-mouse IL-6 (MP5-20F3).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7061	Fluorescein isothiocyanate (FITC) anti-mouse IL-6 (Interleukin-6, IL6)	488	518	IC Flow
12-7061	PE anti-mouse IL-6 (Interleukin-6, IL6)	488	575	IC Flow
14-7061	Affinity Purified anti-mouse IL-6 (Interleukin-6, IL6)	N/A	N/A	ELISA cap ELISPOT cap NU
16-7061	Functional Grade* Purified anti-mouse IL-6 (Interleukin-6, IL6)	N/A	N/A	ELISPOT cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MP5-20F3 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-6 bioactivity, IHC, and intracellular staining for flow cytometric analysis.

Applications Tested

This MP5-20F3 antibody is offered in 2 formats:

- μg size: has been tested by intracellular staining with flow cytometric analysis. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis. This can be used at 20 μl per million cells in a 100 μl total staining volume. The pre-titrated test size contains 0.2% BSA.

References

Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." J Immunol Methods 166(2): 201-14.
Starnes, H. F., Jr., M. K. Pearce, et al. (1990). "Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-alpha challenge in mice" J Immunol 145(12): 4185-91.

Related Products

Cat. 11-4301    FITC Rat IgG1 Isotype Control
Cat. 13-7062    Biotin anti-mouse IL-6 (Interleukin-6, IL6) (clone MP5-32C11)
Cat. 14-8061    Recombinant Mouse IL-6 (Interleukin-6, IL6)
Cat. 00-8222    eBioscience IC Fixation Buffer
Cat. 00-8333    eBioscience Permeabilization Buffer(10X)
Cat. 88-8823    eBioscience Fixation & Permeabilization