Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse interleukin-4 (IL-4)
Catalog Number: 11-7042
Sizes: 25 tests, 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BVD6-24G2
Isotype: Rat IgG1, κ

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with PE anti-mouse CD4 (GK1.5) and intracellularly stained with FITC anti-mouse IL-4 (BVD6-24G2).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7042	FITC anti-mouse IL-4 (Interleukin-4, IL4)	488	518	IC Flow
13-7042	Biotin anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA det ELISPOT
14-7042	Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	IC Flow
25-7042	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IL-4 (Interleukin-4, IL4)	488	760	IC Flow

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Description

The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. BVD6-24G2 has been reported for use in intracellular flow cytometric analysis.

Applications Tested

This BVD6-24G2 antibody is offered in 2 formats:

- μg size: has been tested by intracellular staining and flow cytometric analysis of restimulated mouse splenocyte cultures. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of restimulated mouse splenocyte cultures. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume. The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.
Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." J Immunol Methods 166(2): 201-14.

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