Contents: Fluorescein isothiocyanate (FITC) anti-human interleukin-1 receptor antagonist (IL-1RA)
Catalog Number: 11-7015
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CRM17
Isotype: Mouse IgG1
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Human peripheral blood mononuclear cells were stimulated with LPS in the presence of Brefeldin A for 24 hours. (Left) The cells were surface stained with PE anti-human CD14 (61D3) and intracellulary stained with FITC mouse IgG1 isotype control. (Right) The cells were surface stained with PE anti-human CD14 and intracellularly stained with FITC anti-human IL-1RA (CRM17).
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Description
The CRM17 antibody reacts with human interleukin-1 receptor antagonist (IL-1RA). IL-1RA is produced by macrophages, monocytes, neutrophils, synovial and dermal fibroblasts. The 17 kDa soluble form of IL-1RA (sIL-1RA) is produced by hepatocytes. IL-1RA inhibits the release of both forms of IL-1, the secretion of IL-2, and the expression of IL-2 receptors on the cell surface. IL-1RA also blocks the stimulation of prostaglandin E2 synthesis in synovial cells and the proliferation of thymocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The CRM17 antibody has been reported for use in intracellular staining with flow cytometric analysis.
Applications Tested
The fluorochrome-conjugated CRM17 antibody has been tested by intracellular staining and flow cytometric analysis of LPS-activated, fixed/permeabilized human peripheral blood mononuclear cells. A recommended range of antibody concentrations for optimal staining is 0.05 - 0.5 ug antibody (or 20 ul of prediluted '25 test' reagents) per 1 million cells in 100 ul staining volume. Please see the eBioscience intracellular staining protocol for further information.
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Human Cellular Antigens Chart