Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse interleukin-1α (IL-1α)
Catalog Number: 11-7011
Sizes: 25 tests, 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: ALF-161
Isotype: Armenian Hamster IgG

Mouse thiogylcolate induced PEC were stimulated with LPS in the presence of Brefeldin A for 22 hours. (Left) The cells were surface stained with FITC anti-mouse CD11b (M1/70) and intracellularly stained with PE rat IgG2b isotype control. (Right) The cells were surface stained with FITC anti-mouse CD11b and intracellularly stained with PE anti-mouse IL-1a (ALF-161).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7011	Fluorescein (FITC) anti-mouse IL-1a (Interleukin-1 alpha, IL1a)	488	518	IC Flow
12-7011	Phycoerythrin (PE) anti-mouse IL-1a (Interleukin-1 alpha, IL1a)	488	575	IC Flow
14-7011	Affinity Purified anti-mouse IL-1a (Interleukin-1 alpha, IL1a)	N/A	N/A	ELISA cap IC Flow NU
16-7011	Functional Grade* Purified anti-mouse IL-1a (Interleukin-1 alpha, IL1a)	N/A	N/A	NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The ALF-161 antibody reacts with mouse interleukin-1α (IL-1α). Mouse IL-1α, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1α is mostly cell-associated, with 23% amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ALF-161 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometry.

Applications Tested

The fluorochrome-conjugated ALF-161 antibody has been tested by intracellular staining for flow cytometric analysis of thioglycloate-elicited, LPS-activated, fixed/permeabilized mouse peritoneal exudate cells. A recommended range of antibody concentrations for optimal staining is 0.25 - 0.75 ug antibody per 1 million cells in 100 ul staining volume. Please see the eBioscience intracellular staining protocol for further information. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Fuhlbrigge, R., et al. 1988. Monoclonal antibodies to murine IL-1alpha. J. Immunol. 141: 2643-50.
Rogers, H.W., et al. 1992. Interleukin 1 participates in the development of anti-Listeria responses in normal and SCID mice. Proc Natl Acad Sci U S A. 89: 1011-5.

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