Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse/human/rat cytochrome c
Catalog Number: 11-6601
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 6H2
Isotype: Mouse IgG1, κ

Intracellular staining of HeLa cells with anti-Cytochrome c (6H2) FITC. Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6601	Affinity Purified anti-Cytochrome c	N/A	N/A	IC Flow IP
11-6601	FITC anti-Cytochrome c	488	518	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 6H2 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested

The 6H2 antibody has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Jemmerson et al., Eur J Immunol, 1991 21: 143-151
Goshorn et al, J Biol Chem 1991 266(4) 2134-42.

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