Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse Qa-2 (Qa2)
Catalog Number: 11-5996
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 69H1-9-9
Isotype: Mouse IgG2a, κ

Staining of C57Bl/6 splenocytes with 0.25 µg of FITC mouse IgG2a isotype control (cat.11-4721) (open histogram) or 0.25 µg of FITC 69H1-9-9 (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5996	Affinity Purified anti-mouse Qa-2 (Qa2)	N/A	N/A	FA FC
11-5996	FITC anti-mouse Qa-2 (Qa2)	488	518	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 69H1-9-9 monoclonal antibody reacts with the mouse Qa-2 molecule, a GPI-linked MHC class Iβ surface molecule. Qa-2 is expressed by mature T and B cells, and its expression level is different depending on the laboratory mouse strain.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 69H1-9-9 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 69H1-9-9 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sharrow, S. O., J. S. Arn, et al. (1989). "Epitope clusters of Qa-2 antigens defined by a panel of new monoclonal antibodies." J Immunol 142(10): 3495-502.

Related Products

Cat. 11-4724 Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control