Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse IgE
Catalog Number: 11-5992
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 23G3
Isotype: Rat IgG1, κ

Surface staining of IGEL cells with anti-mouse IgE (23G3) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5992	Affinity Purified anti-mouse IgE	N/A	N/A	ELISA FC
13-5992	Biotin anti-mouse IgE	N/A	N/A	ELISA FC
11-5992	FITC anti-mouse IgE	488	518	FC
12-5992	PE anti-mouse IgE	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 23G3 monoclonal antibody reacts with the ε heavy chain of mouse IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 23G3 has been reported for use in ELISA and flow cytometric analysis.

Applications Tested

This 23G3 antibody has been tested by ELISA and can be used at 1:5000-1:10000. This 23G3 antibody has also been tested by flow cytometric analysis of IGEL cells, and can be used at less than or equal to 1 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Keegan AD, Fratazzi C, Shopes B, Baird B, Conrad DH. 1991. Characterization of new rat anti-mouse IgE monoclonals and their use along with chimeric IgE to further define the site that interacts with Fc epsilon RII and Fc epsilon RI. Mol Immunol. 28(10):1149-54.

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