Contents: Fluorescein isothiocyanate (FITC) anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor)
Catalog Number: 11-5943
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio7H22-E16
Isotype: Rat IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
 
Staining of BALB/c splenocytes with PE anti-mouse CD19 (1D3) (cat. 12-0193) and 0.125 μg of FITC Rat IgG1 Iso Cntrl (cat. 11-4301) (left) or 0.125 μg of FITC anti-mouse BAFF R (eBio7H22-E16) (right). Cells in the lymphocyte gate were used for analysis.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5943 |
Fluorescein isothiocyanate (FITC) anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor) |
488 |
518 |
FC
|
| 51-5943 |
Alexa Fluor® 647 anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor) |
633 |
668 |
FC
|
| 14-5943 |
Affinity Purified anti-mouse BAFF R (BR3, CD268, BLyS receptor 3, BAFF-R, BAFF Receptor) |
N/A |
N/A |
FC
WB
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The monoclonal antibody eBio7H22.E16 reacts with mouse BAFF-Receptor, a type III transmembrane TNF receptor family member. BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice. The receptor is found on the surface of all B220+ B cells found in spleen. BAFF-R is expressed on activated/memory subsets of T-cells and is important for splenic B cell maturation and survival and is a major mediator of BAFF- dependent costimulatory responses in peripheral B and T cells. BAFF-R can cause a high level of autoimmune disease and a defect in BAFF-R will cause a decrease in generating mature B-Cells The ligand for the BAFF-R is BAFF also known as BLyS.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio7H22-E16 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio7H22-E16 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ng LG, Ng CH, Woehl B, Sutherland AP, Huo J, Xu S, Mackay F, Lam KP. BAFF costimulation of Toll-like receptor-activated B-1 cells.
Eur J Immunol. 2006 Jul;36(7):1837-46. (7H22-E16, FC
Pubmed)
Ng LG, Sutherland AP, Newton R, Qian F, Cachero TG, Scott ML, Thompson JS, Wheway J, Chtanova T, Groom J, Sutton IJ, Xin C, Tangye SG, Kalled SL, Mackay F, Mackay CR. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells. J Immunol. 2004 Jul 15;173(2):807-17.(7H22-E16, FC
PubMed)
Thompson JS, Bixler SA, Qian F, Vora K, Scott ML, Cachero TG, Hession C, Schneider P, Sizing ID, Mullen C, Strauch K, Zafari M, Benjamin CD, Tschopp J, Browning JL, Ambrose C. BAFF-R, a newly identified TNF receptor that specifically interacts with BAFF.
Science. 2001 Sep 14;293(5537):2108-11
Related Products
Cat.
11-4301 FITC Rat IgG1 Isotype Control
Mouse Cellular Antigens Chart