Contents: Fluorescein isothiocyanate (FITC) anti-mouse NK1.1
Catalog Number: 11-5941
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
LIGHT-SENSITIVE MATERIAL
DO NOT FREEZE.
Clone: PK136
Isotype: Mouse IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
 
Staining of C57Bl/6 splenocytes with PE anti-mouse CD3e (145-2C11) (cat. 12-0031) and 0.25 μg of FITC Mouse IgG2a, K Iso Cntrl (cat. 11-4724) (left) or 0.25 μg of FITC anti-mouse NK-1.1 (PK136) (right). Cells in the lymphocyte gate were used for analysis.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5941 |
FITC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
488 |
518 |
FC
|
| 12-5941 |
PE anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
488 |
575 |
FC
|
| 13-5941 |
Biotin anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
N/A |
N/A |
FC
|
| 14-5941 |
Affinity Purified anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
N/A |
N/A |
FC
IP
|
| 16-5941 |
Functional Grade* Purified anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
N/A |
N/A |
FC
|
| 17-5941 |
APC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
633 |
660 |
FC
|
| 25-5941 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
488 |
760 |
FC
|
| 45-5941 |
PerCP-Cy5.5 anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
488 |
690 |
FC
|
| 57-5941 |
Pacific Blue® anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No.
14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5
+PK136
- and DX5
-PK136
+ cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. PK136 has been reported for use in flow cytometric analysis.
Applications Tested
The PK136 antibody has been tested by flow cytometric analysis of C57BL/6 mouse splenocyte suspensions and can be used at less than or equal to 0.5 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Koo, G. C. and J. R. Peppard (1984). "Establishment of monoclonal anti-Nk-1.1 antibody."
Hybridoma 3(3): 301-3.
Kitaichi N, Kotake S, Morohashi T, Onoe K, Ohno S, Taylor AW. Diminution of experimental autoimmune uveoretinitis (EAU) in mice depleted of NK cells.
J Leukoc Biol. 2002 Dec;72(6):1117-21. (
PK136, in vivo depletion,
PubMed)
Related Products
Cat.
11-4724 Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control
Mouse Cellular Antigens Chart