Contents: Fluorescein isothiocyanate (FITC) anti-mouse Ly-6G (Gr-1, Gr1)
Catalog Number: 11-5931
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RB6-8C5
Isotype: Rat IgG2b, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of mouse bone marrow cells with PE anti-CD45R/B220 (cat.12-0452) and 0.25 µg of FITC rat IgG2b isotype control (cat.11-4031) (left) or 0.03 µg of FITC RB6-8C5 (right). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5931 |
FITC anti-mouse Ly-6G (Gr-1, Gr1) |
488 |
518 |
FC
|
| 12-5931 |
PE anti-mouse Ly-6G (Gr-1, Gr1) |
488 |
575 |
FC
|
| 13-5931 |
Biotin anti-mouse Ly-6G (Gr-1, Gr1) |
N/A |
N/A |
FC
|
| 14-5931 |
Affinity Purified anti-mouse Ly-6G (Gr-1, Gr1) |
N/A |
N/A |
FA
FC
IH/F
IHC(Paraffin)
IP
WB
|
| 15-5931 |
PE-Cy5 anti-mouse Ly-6G (Gr-1, Gr1) |
488 |
670 |
FC
|
| 16-5931 |
Functional Grade* Purified anti-mouse Ly-6G (Gr-1, Gr1) |
N/A |
N/A |
FA
FC
|
| 17-5931 |
Allophycocyanin (APC) anti-mouse Ly-6G (Gr-1, Gr1) |
633 |
660 |
FC
|
| 19-5931 |
Cy5 anti-mouse Ly-6G (Gr-1, Gr1) |
633 |
670 |
FC
|
| 25-5931 |
PE-Cy7 anti-mouse Ly-6G (Gr-1, Gr1) |
488 |
760 |
FC
|
| 27-5931 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse Ly-6G (Gr-1, Gr1) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 35-5931 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse Ly-6G (Gr-1, Gr1) |
488 |
690 |
FC
|
| 36-5931 |
Functional Grade* Biotin anti-mouse Ly-6G (Gr-1, Gr1) |
N/A |
N/A |
FC
|
| 45-5931 |
PerCP-Cy5.5 anti-mouse Ly-6G (Gr-1, Gr1) |
488 |
690 |
FC
|
| 51-5931 |
Alexa Fluor® 647 anti-mouse Ly-6G (Gr-1, Gr1) |
633 |
668 |
FC
|
| 53-5931 |
Alexa Fluor® 488 anti-mouse Ly-6G (Gr-1, Gr1) |
488 |
519 |
FC
|
| 56-5931 |
Alexa Fluor® 700 anti-mouse Ly-6G (Gr-1, Gr1) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-5931 |
Pacific Blue® anti-mouse Ly-6G (Gr-1, Gr1) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G and Ly-6C, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The RB6-8C5 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Hestdal, K., F. W. Ruscetti, et al. (1991). "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells."
J Immunol 147(1): 22-8.
Fleming, T. J., M. L. Fleming, et al. (1993). "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family."
J Immunol 151(5): 2399-408.(RB6-8C5; Cross-reactivity to Ly-6G and Ly-6C;
PubMed)
Sasmono, R.T., A. Ehrnsperger, et al. (2007). "Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1."
J Leukocyte Biol 82: 111-23.(RB6-8C5; Cross-reactivity to Ly-6G and Ly-6C;
Pubmed)
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Cat.
11-4031 Fluorescein isothiocyanate (FITC) Rat IgG2b Isotype Control
Mouse Cellular Antigens Chart