Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse NKG2A/C/E
Catalog Number: 11-5896
Sizes: 10 ug, 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 20d5
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with PE anti-mouse CD3e (145-2C11) (cat. 12-0031) and 0.25 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (left) or 0.25 μg of FITC 20d5 (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5896	Affinity Purified anti-mouse NKG2A/C/E	N/A	N/A	FC
16-5896	Functional Grade* Purified anti-mouse NKG2A/C/E	N/A	N/A	FC
13-5896	Biotin anti-mouse NKG2A/C/E	N/A	N/A	FC
11-5896	FITC anti-mouse NKG2A/C/E	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 20d5 monoclonal antibody reacts with the mouse NKG2A, C, and E. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD94 and are responsible for recognition of non-classical MHC class I antigen Qa-1.

When co-staining with NKG2A^B6 clone 16a11, it is important to stain for NKG2A^B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 20d5 has been reported for use in flow cytometric analysis.

Applications Tested

The 20d5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.125 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Fraser KP, Gays F, Robinson JH, van Beneden K, Leclercq G, Vance RE, Raulet DH, Brooks CG. 2002. NK cells developing in vitro from fetal mouse progenitors express at least one member of the Ly49 family that is acquired in a time-dependent and stochastic manner independently of CD94 and NKG2. Eur J Immunol. 32:868-78.
Vance RE, Jamieson AM, Cado D, Raulet DH. 2002. Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation. Proc Natl Acad Sci U S A. 99:868-73.
Kraft JR, Vance RE, Pohl J, Martin AM, Raulet DH, Jensen PE. 2000. Analysis of Qa-1(b) peptide binding specificity and the capacity of CD94/NKG2A to discriminate between Qa-1-peptide complexes. J Exp Med. 192:613-24.
Vance RE, Jamieson AM, Raulet DH. 1999. Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells. J Exp Med. 190:1801-12.
Vance RE, Kraft JR, Altman JD, Jensen PE, Raulet DH. 1998. Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b). J Exp Med. 188:1841-8.
Vance RE, Tanamachi DM, Hanke T, Raulet DH. 1997. Cloning of a mouse homolog of CD94 extends the family of C-type lectins on murine natural killer cells. Eur J Immunol. 27:3236-41

Related Products

Cat. 11-4321 FITC Rat IgG2a Isotype Control