Contents: Fluorescein isothiocyanate (FITC) anti-mouse C1qRp (Early B lineage antigen)
Catalog Number: 11-5892
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: AA4.1
Isotype: Rat IgG2b, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 bone marrow cells with PE anti-mo/hu B220 (RA3-6B2) (cat. 12-0452) and 0.5 μg of FITC Rat IgG2b Iso Cntrl (cat. 11-4031) (left) or 0.5 μg of FITC AA4.1 (right). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-5892 |
Affinity Purified anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) |
N/A |
N/A |
FC
IHC
|
| 13-5892 |
Biotin anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) |
N/A |
N/A |
FC
|
| 11-5892 |
FITC anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) |
488 |
518 |
FC
|
| 12-5892 |
PE anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) |
488 |
575 |
FC
|
| 17-5892 |
APC anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) |
633 |
660 |
FC
|
| 25-5892 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) |
488 |
760 |
FC
|
| 45-5892 |
PerCP-Cy5.5 anti-mouse CD93 (AA4.1, C1qRp, Early B lineage antigen) |
488 |
690 |
FC
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The AA4.1 monoclonal antibody reacts with mouse C1qRp, an approximately 130-140 kDa C-type lectin-like type I transmembrane protein. The AA4.1 antigen was originally identified as an antigen expressed on early stages of B cell development in bone marrow. Adult spleen and fetal liver also have detectable numbers of AA4.1
+ cells. In combination with other markers of hematopoietic progenitor cells such as Thy-1, Sca-1, c-Kit, CD43, and CD24, the bone marrow Lymphoid-Committed Progenitors (CLP) can be segregated into more primitive and more differentiated subsets based on expression of AA4.1. Correlated expression of surface IgM (sIgM), CD23, and AA4.1 antigen has also been used to define three nonproliferative subpopulations of immature/transitional peripheral B cells designated: T1 (AA4.1
+/CD23
-/sIgMhi), T2 (AA4.1
+/CD23
+/sIgMhi), and T3 (AA4.1
+/CD23
+/sIgMlo). AA4.1 is also reported to be expressed in cytoplasmic vesicles in endothelial cells, megakaryoblasts, and platelets. It is reported that monoclonal antibodies AA4.1 and 493 recognize different epitopes of the same molecule.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The AA4.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The AA4.1 antibody has been tested by flow cytometric analysis of mouse bone marrow and splenocyte cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Dean YD, McGreal EP, Akatsu H, Gasque P. 2000 Molecular and cellular properties of the rat AA4 antigen, a C-type lectin-like receptor with structural homology to thrombomodulin. J Biol Chem. Nov 3;275(44):34382-92.
McKearn JP, Baum C, Davie JM. 1984 Cell surface antigens expressed by subsets of pre-B cells and B cells. J Immunol. Jan;132(1):332-9.
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Cat.
11-4031 Fluorescein isothiocyanate (FITC) Rat IgG2b Isotype Control
Mouse Cellular Antigens Chart