Contents: Fluorescein isothiocyanate (FITC) anti-human AITR Ligand
Catalog Number: 11-5876
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: EB11
Isotype: Mouse IgG1, κ
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Staining of normal human peripheral blood cells with APC anti-human CD14 (61D3) (cat. 17-0149) and FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (left) or FITC anti-human GITR Ligand (EB11) (right). Cells in the monocyte gate were used for analysis.
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Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The EB11 monoclonal antibody reacts with human Activation-Induced TNFR family member ligand (AITRL), also known as TL6. AITRL is a type II transmembrane protein of the TNF superfamily. AITRL is expressed by endothelial cells and peripheral blood monocytes. Interaction of AITR with AITRL is important for cross-talk between T lymphocytes and endothelial cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The EB11 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This EB11 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 20 μl (1 μg) per million cells in a 100 μl total staining volume.
References
Kwon B, Yu KY, Ni J, Yu GL, Jang IK, Kim YJ, Xing L, Liu D, Wang SX, Kwon BS. 1999. J Biol Chem. 274(10):6056-61. Identification of a novel activation-inducible protein of the tumor necrosis factor receptor superfamily and its ligand.
Related Products
Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control
