Also known as: Endothelial cell-Selective Adhesion Molecule
RUO: For Research Use Only
Staining of the bEnd.3 cell line with 0.25 µg of Rat IgG2a κ Isotype Control FITC (cat. 11-4321) (open histogram) or 0.25 µg of Anti-Mouse ESAM FITC (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse ESAM FITC Catalog Number: 11-5852 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 1G8 Host/Isotype: Rat IgG2a, κ
Product Options
Cat. No.
Size
Price
Add Qty to Cart
11-5852-81
50 ug
11-5852-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 1G8 monoclonal antibody reacts with mouse ESAM (endothelial cell-selective adhesion molecule). ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 1G8 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 1G8 antibody has been tested by fluorometric analysis. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Nasdala I, Wolburg-Buchholz K, Wolburg H, Kuhn A, Ebnet K, Brachtendorf G, Samulowitz U, Kuster B, Engelhardt B, Vestweber D, Butz S. A transmembrane tight junction protein selectively expressed on endothelial cells and platelets.
J Biol Chem. 2002 May 3;277(18):16294-303. Epub 2002 Feb 14. (1G8, FC, IP, IH/F PubMed)
Wegmann F, Petri B, Khandoga AG, Moser C, Khandoga A, Volkery S, Li H, Nasdala I, Brandau O, Fassler R, Butz S, Krombach F, Vestweber D. ESAM supports neutrophil extravasation, activation of Rho, and VEGF-induced vascular permeability. J Exp Med. 2006 Jul 10;203(7):1671-7. Epub 2006 Jul 3. (PubMed)
Wegmann F, Ebnet K, Du Pasquier L, Vestweber D, Butz S. Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts. Exp Cell Res. 2004 Oct 15;300(1):121-33. (PubMed)
