Contents: Fluorescein isothiocyanate (FITC) anti-mouse Ly-49G2 (Ly49G2, LGL-1)
Catalog Number: 11-5781
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio4D11 (4D11)
Isotype: Rat IgG2a, κ
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Staining of C57Bl/6 splenocytes with PE anti-mouse pan-NK (DX5) (cat. 12-5971) and 0.125 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (left) or 0.125 μg of FITC anti-mouse Ly-49G2 (4D11) (right). Total viable cells were used for analysis.
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Description
The 4D11 monoclonal antibody reacts with Ly-49G2 (also known as LGL-1). The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains, which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor. Ly-49G2 is an inhibitory receptor expressed on subsets of natural killer (NK) cells, NK-T cells (in all strains tested), and on a population of memory CD8+ T cells (in C57BL/6 mice). The 4D11 antibody has been reported to cross react with the Ly-49AB6, Ly-49ABALB, and Ly-49T129/J inhibitory receptors and Ly-49LCBA/J activating
receptors. The proportion of NK-T cells expressing Ly-49A and Ly-49G2 is higher in the thymus than in liver, and there is evidence that down-regulation of Ly-49 receptor expression is necessary for normal NK–T-cell development to occur.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio4D11 (4D11) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio4D11 (4D11) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Mason, L.H., et al. 1995. Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2). J. Exp. Med. 182: 293 – 303.
Ortaldo, J.R., et al. 1998. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J. Immunol. 160: 1158 - 1165.
Mason, L., et al. 1988. LGL-1: a non-polymorphic antigen expressed on a major population of
mouse natural killer cells. J. Immunol. 140: 4403 – 4412.
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