Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human Nanog
Catalog Number: 11-5769
Sizes: 25 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: hNanog.1
Isotype: Mouse IgG1, κ

Staining of fixed and permeabilized NTERA cells with 0.25 μg of FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (open histogram) or 0.25 μg of FITC hNanog.1 (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5769	Affinity Purified Mouse anti-human Nanog monoclonal	N/A	N/A	IHC(Paraffin) WB
13-5769	Biotin Mouse anti-human Nanog	N/A	N/A	IC Flow
11-5769	Fluorescein isothiocyanate (FITC) Mouse anti-human Nanog	488	518	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The hNanog.1 antibody was generated against and reacts with human Nanog, a homeobox transcription factor. In addition to the known Oct4 and Stat3 transcriptional operating systems, Nanog plays a crucial role in the second embryonic cell fate specification following formation of the blastocyst. Nanog was identified as a gene specifically expressed in mouse ES cells and preimplantation embryos and is capable of maintaining ES cell self-renewal independently of LIF/Stat3. Potential cross-reactivity of this antibody to other proteins has not been determined.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This hNanog.1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This hNanog.1 antibody has been pre-titrated and tested by flow cytometric analys of NTERA embryonic stem cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S. 2003. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 113(5):631-42.
Cavaleri F, Scholer HR. 2003. Nanog: a new recruit to the embryonic stem cell orchestra. Cell. 113(5):551-2

Related Products

Cat. 11-5768 Fluorescein isothiocyanate (FITC) Mouse anti-human Nanog (clone hNanog.2)
Cat. 14-5768 Affinity Purified Mouse anti-human Nanog monoclonal (clone hNanog.2)