Contents: Fluorescein isothiocyanate (FITC) anti-mouse Ea52-68 peptide bound to the I-A(b)/MHC Class II
Catalog Number: 11-5741
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioY-Ae (YAe, Y-Ae)
Isotype: Mouse IgG2b
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Staining of peripheral blood from a mouse strain known to express the I-A(b)/MHC Class II:Ea peptide complex with PE anti-mo/hu B220 (RA3-6B2) (cat. 12-0452) and 0.25 μg of FITC Mouse IgG2b Iso Cntrl (cat. 11-4732) (left) or 0.25 μg of FITC anti-mouse Ea52-68 peptide (eBioY-Ae) (right). Cells in the lymphocyte gate were used for analysis.
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The Y-Ae antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52-68) bound to I-Ab molecules. The Y-Ae antibody detects a determinant expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb. This determinant is expressed on peripheral B cells and on cells in the thymic medulla, but not thymic cortical epithelium. In addition to B cells, the Y-Ae determinant is expressed at comparable levels on other antigen presenting cells, including macrophages and dendritic cells. The antibody does not react with invariant chain-associated class II MHC complexes.
The reactivity of the Y-Ae antibody is MHC-restricted. The Y-Ae antibody was generated by immunization of [B10.MBR x B10.D2]F1 recipients with B10A(5R) LPS blasts. In this strain combination, there is incompatibility for I-Ab and I-Eb molecules. The antibody reacts with 10-15% of surface I-Ab molecules in strains that also express surface I-Eb molecules (e.g., strains B10.A(5R) and B10.A(3R)). The antibody does not react with I-A molecules in strains that have a non-functional I-E (a chain) gene (e.g., strains B6 and B10), nor with mutant I-A molecules (e.g., (B6.c-H-2bm-12 x A.TFR5)F1), nor with any other strain tested. The Y-Ae antibody recognizes an Ea peptide (Ea 52-68) bound to I-Ab molecules, but not to Ea 52-68 peptide bound to I-Ak or I-Abm-12 molecules.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioY-Ae (YAe, Y-Ae) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioY-Ae (YAe, Y-Ae) antibody has been tested flow cytometric analysis of mouse peripheral blood cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Murphy DB, Lo D, Rath S, Brinster RL, Flavell RA, Slanetz A, Janeway CA Jr.
A novel MHC class II epitope expressed in thymic medulla but not cortex.
Nature. 1989 Apr 27;338(6218):765-8. (
Y-Ae, FC, WB, IHC,
PubMed)
Rudensky AYu, Rath S, Preston-Hurlburt P, Murphy DB, Janeway CA Jr.
On the complexity of self.
Nature. 1991 Oct 17;353(6345):660-2. (
Y-Ae, FC, WB,
PubMed)
Oono T, Fukui Y, Masuko S, Hashimoto O, Ueno T, Sanui T, Inayoshi A, Noda M, Sata M, Sasazuki T.
Organ-specific autoimmunity in mice whose T cell repertoire is shaped by a single antigenic peptide.
J Clin Invest. 2001 Dec;108(11):1589-96. (
Y-Ae, FC, IHC,
PubMed)
Related Products
Cat. 11-4732 FITC Mouse IgG2b Isotype Control
Mouse Cellular Antigens Chart