Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human ILT4 (CD85d) blocking
Catalog Number: 11-5148
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 27D6
Isotype: Rat IgM

Staining of normal human peripheral blood cells with 0.5 μg of FITC Rat IgM Iso Cntrl (cat. 11-4341) (open histogram) or FITC anti-human ILT4 (CD85d) (27D6) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5148	Fluorescein isothiocyanate (FITC) anti-human ILT4 (CD85d) blocking	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 27D6 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leuokocyte Ig-like receptor) MIR (monocytes Ig-like receptor) family. CD85d is a 110 kDa single transmembrane receptor containing 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection. The monoclonal antibody 27D6 has been shown to block binding of ligand (HLA-G tetramer).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 27D6 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 27D6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Colonna M, Samaridis J, Cella M, Angman L, Allen RL, O'Callaghan CA, Dunbar R, Ogg GS, Cerundolo V, Rolink A.Human myelomonocytic cells express an inhibitory receptor for classical and nonclassical MHC class I molecules. J Immunol. 1998 Apr 1;160(7):3096-100.
Allan DS, Colonna M, Lanier LL, Churakova TD, Abrams JS, Ellis SA, McMichael AJ, Braud VM. Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells. J Exp Med. 1999 Apr 5;189(7):1149-56. (27D6, FA, PubMed)
Colonna M, Nakajima H, Navarro F, López-Botet M.A novel family of Ig-like receptors for HLA class I molecules that modulate function of lymphoid and myeloid cells. J Leukoc Biol. 1999 Sep;66(3):375-81(27D6, PubMed)
Manavalan JS, Rossi PC, Vlad G, Piazza F, Yarilina A, Cortesini R, Mancini D, Suciu-Foca N. High expression of ILT3 and ILT4 is a general feature of tolerogenic dendritic cells. Transpl Immunol. 2003 Jul-Sep;11(3-4):245-58. (42D1, FA,FC, PubMed)

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