Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-rat IgG1
Catalog Number: 11-4814
Sizes: 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: R1-3G1
Isotype: Mouse IgG1, κ

3-day Concanavalin A-stimulated C57BL/6 splenocytes were stained with staining buffer alone (red line), or purified rat IgG1 anti-mouse CD25, clone PC61.5 (cat. 14-0251), followed by FITC rat polyclonal anti-rat IgG (cat. 11-4811) (blue line) or isotype specific FITC anti-rat IgG1, clone R1-3G1 (green line). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-4814	Fluorescein isothiocyanate (FITC) anti-rat IgG1	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The R1-3G1 monoclonal antibody can be used as a second step to detect staining with rat IgG1 monoclonal antibodies.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This R1-3G1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This R1-3G1 antibody has been tested by flow cytometric analysis of mouse splenocytes stained with rat IgG1 monoclonal antibodies. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control