Contents: Fluorescein isothiocyanate (FITC) mouse anti-rat IgG (H+L)
Catalog Number: 11-4811
Formulation: Phosphate buffer pH 7.6, 250 mM NaCl, 0.05% NaN3, 1.5% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-4811 |
FITC Anti-Rat IgG |
488 |
518 |
FC
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Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
This antibody will recognize the light chains of other rat immunoglobulins. Reactions to non-immunoglobulin rat serum proteins have not been detected.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in detection of purified rat immunoglobulins (IgG) in flow cytometry.
Applications Tested
FITC Anti-Rat IgG has been tested by flow cytometric analysis to detect purified rat monoclonal antibodies. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, horse, mouse and rabbit serum proteins, but may cross-react with immunoglobulins from other species.
Related Products
Cat. 13-4813 Biotin Anti-Rat IgG (clone Polyclonal)
