Staining of C57BL/6 splenocytes with Anti-Mouse CD11b APC (cat. 17-0112) and 0.25 µg of Rat IgG2a κ Isotype Control FITC (cat. 11-4321) (left) or 0.25 µg of Anti-Mouse F4/80 Antigen FITC (right). Total viable cells were used for analysis.
Contents: Anti-Mouse F4/80 Antigen FITC Catalog Number: 11-4801 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: BM8 Host/Isotype: Rat IgG2a, κ
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Cat. No.
Size
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11-4801-81
50 ug
11-4801-82
100 ug
11-4801-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 125 kDa transmembrane protein. The F4/80 antigen is expressed by a majority of mature macrophages and is the best marker for this population of cells. However, other cell types such as Langerhans cells and liver Kupffer cells have been reported to express this antigen. Expression of F4/80 commences during early myeloid development and is upregulated on all BM cells stimulated in vitro with M-CSF. It has been shown that some cytokines downregulate the expression of F4/80 resulting in lack of F4/80 antigen on a subpopulation of macrophages, especially in the lymphoid microenvironment in vivo.
Applications Reported
For research use only, not for diagnostic or therapeutic use. BM8 has been reported for use in flow cytometric analysis.
Applications Tested
The BM8 antibody has been tested by flow cytometric analysis of mouse spleen and bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Schaller E, Macfarlane AJ, Rupec RA, Gordon S, McKnight AJ, Pfeffer K. 2002. Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol. 22(22):8035-43.
Leenen PJ, de Bruijn MF, Voerman JS, Campbell PA, van Ewijk W. 1994. Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods. 174(1-2):5-19.
Zwadlo G, Brocker EB, von Bassewitz DB, Feige U, Sorg C. 1985. A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. J Immunol. 134(3):1487-92.
Murayama, T., M. Yokode, et al. 1999. Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation. 99(13):1740-6. (IHC frozen, PubMed)
Geutskens, S., T. Otonkoski, et al. 2005. Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro. J Leukoc Biol. 78(4): 845-52. (IHC frozen, PubMed)
Torzewski, M., P. Shaw, et al. 2004. Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization. Aterioscler Thromb Vasc Biol. 24(12): 2307-12. (IHC paraffin, PubMed)
Mackler, A., L.M. Green, et al. 2000. Distribution and activation of uterine mononuclear phagocytes in peripartum endometrium and myometrium of the mouse. Biol Reprod. 62(5):1193-200. (IHC paraffin, PubMed)
