*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
This is a monoclonal mouse IgG2a, κ antibody. It is used as an isotype control for mouse IgG2a antibodies.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This mouse IgG2a isotype control has been reported for use in cell surface and intracellular flow cytometric analysis.
Applications Tested
The FITC conjugated mouse IgG2a isotype control is offered in 2 formats:
- ug size: has been tested by flow cytometric analysis of human peripheral blood leukocytes and can be used at less than or equal to 1 μg/million cells (100 μl blood). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. Please refer to the antibody vial for the recommended test volume to use for one million cells in a 100 μl total staining volume. The pre-titrated test size contains 0.2% BSA.
