Product Information
Contents: Fluorescein isothiocyanate (FITC) Mouse IgG1, κ Isotype Control
Catalog Number: 11-4714
Sizes: 25 tests, 100 tests, 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Isotype: Mouse IgG1, κ
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|
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-4714 |
Affinity Purified Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
IC Flow
IP
WB
|
| 16-4714 |
Functional Grade* Purified Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
|
| 13-4714 |
Biotin Mouse IgG1, K Isotype Control |
N/A |
N/A |
FC
|
| 11-4714 |
Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control |
488 |
518 |
FC
|
| 12-4714 |
Phycoerythrin (PE) Mouse IgG1, K Isotype Control |
488 |
575 |
FC
|
| 15-4714 |
Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control |
488 |
670 |
FC
|
| 17-4714 |
Allophycocyanin (APC) Mouse IgG1, K Isotype Control |
633 |
660 |
FC
|
| 25-4714 |
Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control |
488 |
760 |
FC
|
| 35-4714 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG1, K Isotype Control |
488 |
690 |
FC
|
| 51-4714 |
Alexa Fluor® 647 Mouse IgG1, K Isotype Control |
633 |
668 |
FC
|
| 53-4714 |
Alexa Fluor® 488 Mouse IgG1, K Isotype Control |
488 |
519 |
FC
|
| 56-4714 |
Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-4714 |
Pacific Blue® Mouse IgG1, K Isotype Control |
402 |
421 |
FC
|
| 27-4714 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 45-4714 |
PerCP-Cy5.5 Mouse IgG1, K Isotype Control |
488 |
690 |
FC
|
| 93-4714 |
eFluorTM 605NC Mouse IgG1, Isotype Control |
350-500 |
605 |
FC
|
| 94-4714 |
eFluorTM 625NC Mouse IgG1 Isotype Control |
350-500 |
625 |
FC
|
| 95-4714 |
eFluorTM 650NC Mouse IgG1, K Isotype Control |
350-500 |
650 |
FC
|
| 47-4714 |
Allophycocyanin-eFluorTM 780 Mouse IgG1, K Isotype Control (APC-Alexa Fluor® 750 replacement) |
633 |
780 |
FC
|
| 48-4714 |
eFluorTM 450 Mouse IgG1, K Isotype Control (Pacific Blue® replacement) |
405 |
450 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The monoclonal mouse IgG1, κ, immunoglobulin is useful as an isotype control.
Applications Reported
For research use only, not for diagnostic or therapeutic use. FITC Mouse IgG1, κ Iso Cntrl has been reported for use in flow cytometric analysis.
Applications Tested
FITC Mouse IgG1, K Iso Cntrl is offered in 2 formats:
- μg size: has been tested . This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested . This can be used at 20 μl (1 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume). The pre-titrated test size contains 0.2% BSA.
