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Fluorescein isothiocyanate (FITC) anti-mouse IgA

 
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Contents: Fluorescein isothiocyanate (FITC) anti-mouse IgA
Catalog Number: 11-4204
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: mA-6E1
Isotype: Rat IgG1, κ

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11-4204-81 50 ug
11-4204-82 100 ug
11-4204-83 200 ug


   

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
11-4204 Fluorescein isothiocyanate (FITC) anti-mouse IgA 488 518 FC 
12-4204 Phycoerythrin (PE) anti-mouse IgA 488 575 FC 
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description


The mA-6E1 monoclonal antibody reacts with the heavy chain of mouse IgA. It does not react with other isotypes of antibody, including IgD, IgG or IgM, or with other species isotypes. IgA, which is mainly found as a dimer through association of the J chain, is found in mucous secretions, but not in plasma. Fluorochrome conjugated mA-6E1 is used as a detection secondary for mouse IgA primary antibodies.


Applications Reported


For research use only, not for diagnostic or therapeutic use. This mA-6E1 antibody has been reported for use in flow cytometric analysis.


Applications Tested


This mA-6E1 antibody has been tested by flow cytometric analysis as a second step to detect mouse IgA primary antibodies. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


Related Products


Cat. 11-4301  FITC Rat IgG1 Isotype Control
Cat. 12-5994  PE anti-mouse IgA (clone 11-44-2)
Cat. 13-5994  Biotin anti-mouse IgA (clone 11-44-2)
Cat. 14-5994  Affinity Purified anti-mouse IgA (clone 11-44-2)


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