Contents: Fluorescein isothiocyanate (FITC) anti-mouse IgA
Catalog Number: 11-4204
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: mA-6E1
Isotype: Rat IgG1, κ
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|
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-4204 |
Fluorescein isothiocyanate (FITC) anti-mouse IgA |
488 |
518 |
FC
|
| 12-4204 |
Phycoerythrin (PE) anti-mouse IgA |
488 |
575 |
FC
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The mA-6E1 monoclonal antibody reacts with the heavy chain of mouse IgA. It does not react with other isotypes of antibody, including IgD, IgG or IgM, or with other species isotypes. IgA, which is mainly found as a dimer through association of the J chain, is found in mucous secretions, but not in plasma. Fluorochrome conjugated mA-6E1 is used as a detection secondary for mouse IgA primary antibodies.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This mA-6E1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This mA-6E1 antibody has been tested by flow cytometric analysis as a second step to detect mouse IgA primary antibodies. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 11-4301 FITC Rat IgG1 Isotype ControlCat. 12-5994 PE anti-mouse IgA (clone 11-44-2)Cat. 13-5994 Biotin anti-mouse IgA (clone 11-44-2)Cat. 14-5994 Affinity Purified anti-mouse IgA (clone 11-44-2)
Mouse Cellular Antigens Chart