Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7)
Catalog Number: 11-1979
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 3D12
Isotype: Rat IgG2a, κ

Staining of normal human peripheral blood cells with APC anti-human CD45RA (HI100) (cat. 17-0458) and 0.5 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (left) or FITC anti-human CCR7 (3D12) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1979	Affinity Purified anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7)	N/A	N/A	FC IHC(Paraffin)
11-1979	Fluorescein isothiocyanate (FITC) anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7)	488	518	FC
12-1979	Phycoerythrin (PE) anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7)	488	575	FC
17-1979	Allophycocyanin (APC) anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7)	633	660	FC
47-1979	Allophycocyanin-eFluor^TM 780 anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7) (APC-Alexa Fluor® 750 replacement)	633	780	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naïve from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC’s. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 3D12 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
It is recommended that the staining incubation time be increased to at least 45 minutes at 4C for optimal staining.

References

Geginat J, Lanzavecchia A, Sallusto F. Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines. Blood. 2003 Jun 1;101(11):4260-6. (3D12, FC, PubMed)

Sallusto F, Lenig D, Forster R, Lipp M, Lanzavecchia A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature. 1999 Oct 14;401(6754):708-12. (3D12, FC, PubMed)

Related Products

Cat. 11-4321 FITC Rat IgG2a Isotype Control