Also known as: Interleukin-8 Receptor A, IL-8Ra, IL8RA
RUO: For Research Use Only
Staining of normal human peripheral blood cells with Anti-Human CD182 (CXCR2) PE (cat. 12-1829) and Mouse IgG2b κ Isotype Control FITC (cat. 11-4732) (left) or Anti-Human CD181 (CXCR1) FITC (right). Cells in the large scatter population were used for analysis.
Contents: Anti-Human CD181 (CXCR1) FITC Catalog Number: 11-1819 Concentration: 20 μl (0.5 μg)/test Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBio8F1-1-4 (8F1-1-4) Host/Isotype: Mouse IgG2b, κ
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Size
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11-1819-71
25 tests
11-1819-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBio8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Rα). CD181 is a 67-70 kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rβ) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-α, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio8F1-1-4 (8F1-1-4) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio8F1-1-4 (8F1-1-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (0.5 μg)/test per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Wolf M, Delgado MB, Jones SA, Dewald B, Clark-Lewis I, Baggiolini M.
Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2.
Eur J Immunol. 1998 Jan;28(1):164-70.
Lippert U, Artuc M, Grutzkau A, Moller A, Kenderessy-Szabo A, Schadendorf D, Norgauer J, Hartmann K, Schweitzer-Stenner R, Zuberbier T, Henz BM, Kruger-Krasagakes S.
Expression and functional activity of the IL-8 receptor type CXCR1 and CXCR2 on human mast cells. J Immunol. 1998 Sep 1;161(5):2600-8.
Li F, Zhang X, Gordon JR.
CXCL8((3-73))K11R/G31P antagonizes ligand binding to the neutrophil CXCR1 and CXCR2 receptors and cellular responses to CXCL8/IL-8.
Biochem Biophys Res Commun. 2002 May 10;293(3):939-44.
Sprenger H, Lloyd AR, Meyer RG, Johnston JA, Kelvin DJ.
Genomic structure, characterization, and identification of the promoter of the human IL-8 receptor A gene.
J Immunol. 1994 Sep 15;153(6):2524-32.
Chuntharapai A, Lee J, Hebert CA, Kim KJ.
Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes.
J Immunol. 1994 Dec 15;153(12):5682-8.
