Contents: Fluorescein isothiocyanate (FITC) anti-human CD154
Catalog Number: 11-1548
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE
LIGHT-SENSITIVE MATERIAL
Clone: 24-31
Isotype: Mouse IgG1, κ
HLDA No.: N/A
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Human PBMC samples were activated with TPA and Ionomycin for 6 hours and stained with 0.5 µg of anti-CD154-FITC. Viable cells were analyzed and gated for small cells. Markers were set based on the autofluorescence sample.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-1548 |
Affinity Purified anti-human CD154 (CD40 Ligand, CD40L) |
N/A |
N/A |
FA
FC
|
| 16-1548 |
Functional Grade* Purified anti-human CD154 (CD40 Ligand, CD40L) |
N/A |
N/A |
FA
FC
|
| 11-1548 |
FITC anti-human CD154 (CD40 Ligand, CD40L) |
488 |
518 |
FC
|
| 12-1548 |
PE anti-human CD154 (CD40 Ligand, CD40L) |
488 |
575 |
FC
|
| 17-1548 |
Allophycocyanin (APC) anti-human CD154 (CD40 Ligand, CD40L) |
633 |
660 |
FC
|
| 27-1548 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD154 (CD40 Ligand, CD40L) |
633 |
779 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the Cynomogus monkey CD154.
Applications Reported
For research use only, not for diagnostic or therapeutic use. 24-31 has been reported for use in flow cytometric analysis.
Applications Tested
The 24-31 antibody has been pretitrated and tested by flow cytometric analysis of resting and activated human PBMC and can be used at 20 µl (0.5 μg) per test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Brams, P., A. Black, et al. 2001. A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation. Int Immunopharmacol 1(2): 277-94.
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11-4718 FITC Mouse IgG1, K Isotype Control (I/C Staining)
Human Cellular Antigens Chart