Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD148 (DEP-1, high cell density-enhanced protein tyrosine phosphatase 1, HPTP-eta, and PTP receptor type J, PTPRJ)
Catalog Number: 11-1489
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A3
Isotype: Mouse IgG1, k

Staining of normal human peripheral blood cells with 0.5 μg of FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (open histogram) or FITC anti-human CD148 (A3) (colored histogram). Cells in the lymphocyte gate (left) or granulocyte gate (right) were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1489	Fluorescein isothiocyanate (FITC) anti-human CD148 (DEP-1, high cell density-enhanced protein tyrosine phosphatase 1, HPTP-eta, and PTP receptor type J, PTPRJ)	488	518	FC

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Description

The monoclonal antibody A3 recognizes human CD148 also known as Dep-1 (density-enhanced protein tyrosine phosphatase) and ByP. CD148 is a highly glycosylated transmembrane protein, up to 250kDa, containing eight Fibronectin Type III domains in the extracellular region and one tyrosine phosphatase domain in the cytoplasmic region. Expression is found broadly including fibroblasts, epithelial cells, lymphocytes (B, T and NK cells), and myeloid cells (monocytes and granulocytes). CD148 expression can be upregulated following T cell activation. The role for CD148 has been shown to involve the dephosphorylation of proteins such as LAT and PLC-γ. One key role is the down regulation of Tcell activation. Recently CD148 has been shown to be excluded from the synapse (junction between T cells and antigen presenting cells APCs). Once the synapse disengages, CD148 can relocate and exert its effects to down-regulate the activated state. Furthermore expression of CD148 has been shown to inhibit the growth of breast cancer cells in vitro.
The monoclonal antibody A3 can neutralize the negative signal from T cell activation thereby causing activation of T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This A3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This A3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Baker JE, Majeti R, Tangye SG, Weiss A. Protein tyrosine phosphatase CD148-mediated inhibition of T-cell receptor signal transduction is associated with reduced LAT and phospholipase Cgamma1 phosphorylation. Mol Cell Biol. 2001 Apr;21(7):2393-403. (A3, FC, PubMed)

Tangye SG, Phillips JH, Lanier LL, de Vries JE, Aversa G. CD148: a receptor-type protein tyrosine phosphatase involved in the regulation of human T cell activation. J Immunol. 1998 Oct 1;161(7):3249-55. (A3, FC, FA, PubMed)

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