Left: Staining of HUVECs with 0.25 μg of FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (blue) or FITC anti-human CD146 (Muc 18, MCAM, Mel-CAM, EndoCAM) (purple). Right: PBMCs were stained with APC CD3 and FITC CD146.
Contents: Fluorescein isothiocyanate (FITC) anti-CD146 Catalog Number: 11-1469 Concentration: 5µl (0.25 µg)/test Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Clone: P1H12 Host/Isotype: Mouse IgG1
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11-1469-41
25 tests
11-1469-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The monoclonal antibody P1H12 recognizes human CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates.
The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This P1H12 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of Human Umbilical Vein Cells (HUVECs). This can be used at 5 μl (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Lamerato-Kozicki AR, Helm KM, Jubala CM, Cutter GC, Modiano JF. Canine hemangiosarcoma originates from hematopoietic precursors with potential for endothelial differentiation. Exp Hematol. 2006 Jul;34(7):870-8. (P1H12, canine cross-reactivity, PubMed)
Elshal MF, Khan SS, Takahashi Y, Solomon MA, McCoy JP Jr. CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood.
Blood. 2005 Oct 15;106(8):2923-4 (P1H12, FC, PubMed)
Solovey AN, Gui L, Chang L, Enenstein J, Browne PV, Hebbel RP. Identification and functional assessment of endothelial P1H12.
J Lab Clin Med. 2001 Nov;138(5):322-31. (P1H12, FC, PubMed)
Solovey A, Lin Y, Browne P, Choong S, Wayner E, Hebbel RP. Circulating activated endothelial cells in sickle cell anemia.
N Engl J Med. 1997 Nov 27;337(22):1584-90. (P1H12, FC PubMed)
