Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra)
Catalog Number: 11-1231
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 5B11
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 bone marrow cells with APC anti-mouse CD11b (M1/70) (cat. 17-0112) and 1.0 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (left) or 1.0 μg of FITC anti-mouse CD123 (5B11) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1231	Affinity Purified anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	N/A	N/A	FC
13-1231	Biotin anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	N/A	N/A	FC
11-1231	Fluorescein isothiocyanate (FITC) anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	488	518	FC
12-1231	PE anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra)	488	575	FC
17-1231	Allophycocyanin (APC) anti-mouse CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra)	633	660	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 5B11 monoclonal antibody reacts with mouse CD123, the α chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself and when associated with either CD131 (common β chain) or AIC2A (IL-3β) binds IL-3 with high affinity. CD123 does not transduce any intracellular signals upon binding IL-3 and requires the β chain for this function.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 5B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 5B11 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspension. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ichihara, M., T. Hara, et al. (1995). "Impaired interleukin-3 (IL-3) response of the A/J mouse is caused by a branch point deletion in the IL-3 receptor alpha subunit gene." Embo J 14(5): 939-50.
Mueller, D. L., Z. M. Chen, et al. (1994). "Subset of CD4+ T cell clones expressing IL-3 receptor alpha-chains uses IL-3 as a cofactor in autocrine growth." J Immunol 153(7): 3014-27.

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Cat. 11-4321 FITC Rat IgG2a Isotype Control