Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD107b (LAMP-2)
Catalog Number: 11-1072
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioABL-93 (ABL-93)
Isotype: Rat IgG2a, κ
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Staining of thioglycolate-induced peritoneal exudate cells with 0.25 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (open histogram) or 0.25 μg of FITC anti-mouse CD107b (ABL-93) (colored histogram). Total viable cells were used for analysis.
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Description
The eBioABL-93 monoclonal antibody reacts with mouse CD107b, also known as LAMP-2 (lysosomal-associated membrane protein 2). CD107b, an approximately 120 kDa protein, is highly glycosylated and expressed intracellularly on myeloid cells and epithelial cells. It is reported that CD107b is identical to the mouse Mac-3 antigen.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioABL-93 (ABL-93) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This eBioABL-93 (ABL-93) antibody has been tested by intracellular staining and flow cytometric analysis of thioglycolate-induced peritoneal-exudate cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Chen JW, Murphy TL, Willingham MC, Pastan I, August JT.
Identification of two lysosomal membrane glycoproteins.
J Cell Biol. 1985 Jul;101(1):85-95. (
ABL-93, IP, IHC,
PubMed)
Cha Y, Holland SM, August JT.
The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins.
J Biol Chem. 1990 Mar 25;265(9):5008-13. (
ABL-93, affinity-chromatography,
PubMed)
Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT.
Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2.
Biochem Soc Symp. 1986;51:97-112.
Related Products
Cat.
11-1078 Fluorescein isothiocyanate (FITC) anti-human CD107b (LAMP-2) (clone eBioH4B4 (H4B4))
Cat.
11-4321 FITC Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart