Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD99 (E2 antigen)
Catalog Number: 11-0997
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 3B2/TA8
Isotype: Mouse IgG1
HLDA No.: VI

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0997	Affinity Purified anti-human CD99 (E2 antigen)	N/A	N/A	FC
11-0997	Fluorescein isothiocyanate (FITC) anti-human CD99 (E2 antigen)	488	518	FC
12-0997	Phycoerythrin (PE) anti-human CD99 (E2 antigen)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 3B2/TA8 monoclonal antibody reacts with human CD99, also known as E2 antigen and MIC2. CD99 is an approximately 32 kDa sialoglycoprotein and is expressed by all leukocytes. CD99 is involved in T cell adhesion and activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 3B2/TA8 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 3B2/TA8 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (1 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

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Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control